Kinetic Studies of Alkaline Phosphatase from the Liver of Agama agama Lizard
نویسندگان
چکیده
Kinetic studies were carried out on alkaline phosphatase (ALP) extracted from the liver of Agama agama lizard. Incubation of ALP extract with para – nitrophenyl phosphate formed the basis for the determination of enzyme activity. Spectrophotometric method was used to assay the enzyme, and the kinetic constants: Maximum velocity (Vmax) and Michaelis Menten constant (Km) were calculated. The Km and Vmax values were 2.5mM and 1.538 X 10-3 μmol/min respectively. Inhibition studies show that the enzyme activity was competitively inhibited by 0.67mM sodium hydrogen orthophosphate (NaH2PO4) with inhibition constant (Ki) of 2.27mM. The optimum pH of the enzyme was 9.9 and the optimum temperature was 35OC. The enzyme exhibited linear Arrhenius relationship with corresponding catalytic energy of activation (Ea) of 1.44KJmol-1. This study gives an insight of the catalytic properties of ALP extracted from the liver of Agama agama lizard.
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